General Function Checklist

Default REST API data sources

Select File -> Preferences -> Data Sources tab.

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• REST API data sources (e.g., UCSC REST and Ensembl REST) appear in the Data Sources table.

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• REST API data sources are all enabled.

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• None of the rows have a red or yellow background.

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Click on the  icon in the Information column for UCSC REST.

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• The data listed in the Value column are specific to the UCSC REST data source.

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Close this information window, then click on the  icon for Ensembl REST.

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• The data listed in the Value column updated appropriately and are specific to the Ensembl REST data source.

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UCSC REST Data Source

1. Disable all REST API data sources except UCSC REST.
2. Close Preferences.
3. Close and re-open IGB.
4. Select File -> Preferences -> Data Sources tab.

• UCSC REST is still the only REST API data source enabled.

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Disable UCSC REST, then re-enable it.

• The UCSC REST row does not have a red or yellow background.

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Open the A_carolinensis_May_2010 genome.

• The UCSC REST data source is present in the Available Data section.

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1. In the Available Data section, expand the UCSC REST folder, then expand the genePred folder.
2. Check the box next to RefSeq All (ncbiRefSeq).
3. Click Load Data.

• Gene models appear and look like those pictured below:

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1. Navigate to chr1:53,657,023-53,657,065
2. Click Load Sequence

• The sequence data loads in the Coordinates track.

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• There are no warnings or errors in the Log.

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