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Note: Soft-clipped sections of reads are not visible in versions of IGB prior to 9.1
To view your own data with soft-clipping within IGB, first load a BAM filein IGB:
- Open Add your BAM file to IGB.
- Zoom in on the gene or region of interest.
- Click Load Data.
To view the example soft-clipping data in the images below:
- Open the human genome (H_sapiens_Dec_2013).
- Navigate to: chr1:3,320-26,768
- Hide the RefSeq Curated annotation track by right-clicking and selecting Hide.
- Select File > Open URL...
- Click Load Sequence to load sequence data.
- Click Load Data to load data into it.
Soft-clipping is enabled by default in IGB, with the soft-clipped section of the read displayed in gray.
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