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Note: Soft-clipped sections of reads are not visible in versions of IGB prior to 9.1

To view your own data with soft-clipping within IGB, first load a BAM filein IGB:

  1. Open Add your BAM file to IGB.
  2. Zoom in on the gene or region of interest.
  3. Click Load Data.

To view the example soft-clipping data in the images below:

  1. Open the human genome (H_sapiens_Dec_2013).
  2. Navigate to: chr1:3,320-26,768
  3. Hide the RefSeq Curated annotation track by right-clicking and selecting Hide.
  4. Select File > Open URL...
    1. Enter: https://quickload-testing.s3.amazonaws.com/smokeTestingQuickload/H_sapiens_Dec_2013/Bam/pacBio.bam
    2. Click OK
  5. Click Load Sequence to load sequence data.
  6. Click Load Data to load data into it.

 

Soft-clipping is enabled by default in IGB, with the soft-clipped section of the read displayed in gray.

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