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  1. Zooming in on the gene or region of interest.
  2. Click on Load Data.
  3. Right-click on the track, and select Track Operations > FindJunctions > Use Default

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  1. .
  2. Click on OK.

 

FindJunctions will appear as a new track. Brackets represent splice junctions across exons. The number of reads supporting each splice junction is shown above each bracket.

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The default behavior for FindJunctions is to identify split reads with a minimum of five base pairs on either side of the exon.

You can change this number the default FindJunctions behavior by:

  1. Right-clicking on the track, and select selecting Track Operations > FindJunctions > Configure….
  2. Inputting a new number in the threshold box (default is 5).

How to get FindJunctions

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