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1. Open the human genome (H_sapiens_Dec_2013) in IGB.
2. Navigate to: chr1:3,320-26,768
3. Hide the RefSeq Curated annotation track by right-clicking and selecting Hide.
4. Select File > Open URL...Enter: https://quickload-testing.s3.amazonaws.com/smokeTestingQuickload/H_sapiens_Dec_2013/Bam/Download the two files below:
   1. pacBio.bam
   2. pacBio.bam.bai
5. In IGB, select File > Open File...
6. Select the pacBio.bam file and click Open.bai
7. Click OK
8. Click Load Sequence to load sequence data.
9. Click Load Data to load data into it.

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Soft-clipping is enabled by default in IGB, with the soft-clipped section of the read displayed in gray.

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1. Right-click the reads track label.
2. Select Configure soft-clip.

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Options for viewing soft-clipping include:

1. Show as default color - Soft-clipping shows as a default gray.
2. Show as custom color... - Using a color picker, soft-clipping shows as selected color.
3. Show as bases - Soft-clipping shows as Residue Colors.
4. Hide soft-clipping - Soft-clipping is hidden.

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Note: When using the hide soft-clipping option, a line showing the full length of the read including the soft-clipped portion will appear.

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• Depth Graph (All) and Depth Graph (Start) ignore the soft-clipped parts of reads.
• Mismatch Graph and Mismatch Pileup Graph include the soft-clipped parts of reads.

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Data used in these examples is a subset taken from the Genome in a Bottle consortium (HG002 PacBio CCS 10kb):

Zook JM, Catoe D, McDaniel J, et al. Extensive sequencing of seven human genomes to characterize benchmark reference materials. Sci Data. 2016;3:160025. Published 2016 Jun 7. doi:10.1038/sdata.2016.25

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