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IGB displays insertions, deletions and mismatches as follows:

  • Insertions - residues Residues flanking insertion site appear are shown in white black with a black backgroundwhite background by default (purple on white here); triangular shapes between them point to the location of the inserted base(s) present in the aligned sequence but not in the reference. 

    • Select an insertion and the Selection Info tab to display the sequence of inserted bases

  • Deletions - residues Residues deleted in the aligned sequenced relative to the reference appear as horizontal lines that are of the same color and connect flanking residues.
  • Mismatches - residues Residues in the aligned sequence that do not match the corresponding base in the reference appear as highlighted bases. To highlight mismatches, click Load Sequence

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