IGB User's Guide
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  • Depth Graph (All) and Depth Graph (Start) ignore the soft-clipped parts of reads.
  • Mismatch Graph and Mismatch Pileup Graph include the soft-clipped parts of reads.

 

Data used in these examples is from the Genome in a Bottle consortium:

Zook JM, Catoe D, McDaniel J, et al. Extensive sequencing of seven human genomes to characterize benchmark reference materials. Sci Data. 2016;3:160025. Published 2016 Jun 7. doi:10.1038/sdata.2016.25