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Creating Graphs
IGB can create several useful graphs from annotation tracks. Although the Depth graph can be utilized for all annotations, the usefulness shines, in combination with the Mismatch graph, with .bam and other short read alignment files. Here, you can see how many reads are present at each nucleotide position (Depth) and where mismatches occur as compared to the genomic sequence file.
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The picture below shows a fully expanded BAM file, showing the difficulty of seeing any details. The second picture shows just the depth graph (*BAM *track is 'hidden') with the tool tip showing the number of reads aligned onto that specific genomic location.
Mismatch Graph
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