General Function Checklist

Objective: Validate that soft-clipping can be hidden and customized using the right-click menu of a bam track, as shown below:

Add the smoke test Data Provider to IGB using the following URL: https://quickload-testing.s3.amazonaws.com/smokeTestingQuickload/

Ensure that loading data looks like this (stack height set at 10, reads may be organized randomly):

 

Validate that the 'Configure soft-clip' submenu of the bam track right-click menu has 'Show as default color' selected.

 

Right-click on the bam track and select:
Configure soft-clip -> Show as custom color

A color picker should appear. Select a color (the example below selected red).

Ensure your view is as below, with the color you chose correctly applied:

 

Right-click on the bam track and select:
Configure soft-clip -> Show as bases

Ensure your view is as below:


Right-click on the bam track and select:
Configure soft-clip -
> Hide softclipping

Ensure your view is as below: 

 


To test track operations are working correctly for soft-clipped data, right-click on the bam track and select:

  1. Track Operations > Depth Graph (All)
  2. Track Operations > Depth Graph (Start)
  3. Track Operations > Mismatch Graph
  4. Track Operations > Mismatch Pileup Graph
  5. Collapse

Ensure your view is as below: