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Introduction
As you learn to use IGB, you'll find it offers many features that make it one of the best tools available for visualization and exploration of genomic data sets.
If you are new to IGB, the following six step Quick Start Guide will help you get started using IGB.
Step 1: Download and start IGB
1. Go to bioviz.org/igb and click Downloads.
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IGB Download - Small, medium, and large memory options
Step 2: Choose species and genome version
Click a start screen (red box) shortcut image.
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IGB after selecting the human genome
Step 3. Open data sets and click Load Data
Choose data sets from Available Data file tree
Open a folder under the Available Data file tree (red box) by clicking on it.
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When you open a data set, IGB adds it as new empty track to the main view and lists it in the Data Management table. |
Click Load Data button to view data
- Zoom or scroll to a region of interest (See Step 5) then
- Click Load Data (green arrow, box in above image) to load data into the IGB main view.
Step 4: Load data from local files
To view data from local or remote files
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Many data files and data sets (such as BAM files) contain too much data to display at once. For this reason, IGB gives you total control over when and how data are loaded. To load and view data, zoom in on a region and click the Load Data button. Regions where data have not yet been loaded have slightly darker backgrounds. |
Loading a reference sequence (optional)
If you have a sequence file (fasta, 2bit, bnib, etc.) that you would like to use as the reference sequence, use the File > Open Reference Sequence... option. After selecting the file, click the Load Data button to load sequence. The sequence data will appear below the Coordinate Axis in the center of the display.
Step 5: Zooming in on a region of interest
If you're working with large files such as .bam, .sam, .wig, .bedGraph, you should 'zoom in' to a smaller area before loading your data.
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- More about scrolling/panning in IGB
- More about the main view
- More about the tabbed panels and about customizing them.
Step 6: Configure tracks
You can change track color, how IGB indicates strand, track labels, track label color and font, track height, annotation stack height, and much more.
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