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Comment: Updated the formatting to be consistent with other smoke testing pages.

General Function Checklist

Objective: Validate that soft-clipping can be hidden and customized using the right-click menu of a bam track, as shown below:

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  1. In the Data Sources tab in Preferences, add the smoke test Data Provider to IGB using the following URL: https://quickload-testing.s3.amazonaws.com/smokeTestingQuickload/
  2. Open

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  1. the H_Sapiens_Dec_2013 genome.
  2. In the Available Data panel, add the Bam - PacBio with

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  1. softclipping data from your newly added Quickload.
  2. Navigate to: chr1:9,971-10,034
  3. Click Load Sequence

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  1. to load sequence data,

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  1. then click Load Data to load data into it.

Ensure that loading Image Added

  • Loading data looks like this (stack height set at 10, reads may be organized randomly):
  •  mac
  •  linux
  •  windows

Image Removed

 



Validate that the 'Image Added

  • The Configure soft-clip

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  • submenu of the bam track right-click menu has

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  • Show as default color

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  • selected.
  •  mac
  •  linux
  •  windows

Image Removed

 



  1. Right-click on the bam track and select:

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  1. Configure soft-clip -> Show as custom color

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A color picker should appear. Select a color (the example below selected red).

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  1. .
  2. Choose a red color from the color picker.

Image Added

  • Your IGB looks like the above image.
  •  mac
  •  linux
  •  windows

Image Removed

 



Right-click on the bam track and select:
Configure soft-clip -> Show as basesEnsure your view is as below:.

Image Added

  • Your IGB looks like the above image.
  •  mac
  •  linux
  •  windows

Image Removed


Right-click on the bam track and select:
Configure soft-clip -> Hide softclippingEnsure your view is as below: .

Image Added

  • Your IGB looks like the above image.
  •  mac
  •  linux
  •  windows

Image Removed 

To test track operations are working correctly for soft-clipped data, right right-click on the bam track and select the following:

  1. Track Operations > Depth Graph (All)
  2. Track Operations > Depth Graph (Start)
  3. Track Operations > Mismatch Graph
  4. Track Operations > Mismatch Pileup Graph
  5. Collapse

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Image Added

  • Your IGB looks like the above image.
  •  mac
  •  linux
  •  windows

Image Removed