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Introduction
As you learn to use IGB, you'll find it offers many features that make it one of the best tools available for visualization and exploration of genomic data sets.
If you are new to IGB, the following use this Quick Start Guide will help you get started.
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Step 1: Get and start IGB
- Go to biovizBioViz.org/igb/download.html IGB Download PageDownload and launch IGB and click the Download button
- Select and download the installer for your platform
If you have trouble starting IGB, see Troubleshooting or ask for help on the Genoviz Help Forum.visit the help page on BioViz.org for assistance.
Step 2: Choose species and genome version
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- Choose Species and Genome Version using the Current Genome tab.
IGB start screen
If your species or genome version is not listed, you can enter it as a custom species and genome when you open a data file. For more information, see view it if you have a fasta or 2bit file with sequence data. See Custom Genomes
Step 3. Open data sets
Open data sets from remote data sources (Data Access tab) or by opening local files.
To open a data set from a remote an IGB Quickload data source (see About IGB Quickload)
- Select Data Access
- Select data sets in the Available Data sub-panel section
To open local files on your computer
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When you select a data sets or a file, IGB will add adds a new empty track to the main view and list lists it in the Data Management table. Regions Empty regions in the new track that do not have data loaded have a darker background colorare gray.
IGB window after opening a data set
Open reference sequence (optional)
If you would like to open a sequence file (fasta or 2bit format) to use as the reference sequence
- Select File > Open Reference Sequence...
- Choose the file
human genome RNA-Seq coverage graphs from Adrenal Gland, Kidney, and Thymus data sets
Step 4: Zoom in
Because many data files contain too much data to view all at once, IGB does not load data into the viewer until you click the Load Data button.
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IGB horizontal zooming controls
Other ways to zoom
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- Search for a gene by name or keyword (For example, TBATA or thymus)
- Double-click a featurean exon or gene model to zoom in on it
- Click-drag within the coordinate axis to zoom in on a region
See also:
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To load data, click Load Data button. Regions with loaded data show the selected background color; areas without loaded data appear darker.
IGB after loading data
See also:
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To change style elements of a track, click the track label and use the Annotation or Graph tab to change to change color, track height, annotation label, amount of data shown (stack height), and other options.
See also: