ProtAnnot

General Function Checklist

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1. Install the ProtAnnot plugin through the IGB App Manager (Tools > Open App Manager)

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   1. 1. It may be helpful to watch this How to use ProtAnnot YouTube video if you are unfamiliar with using ProtAnnot.
   2. Open the A_thaliana_Jun_2009 genome.
   3. Navigate to the following gene: Chr1:2,257,247-2,260,234
   4. Select the AT1G07350.1 and AT1G07350.2 gene models by shift-clicking both models. Make sure that the full model is selected, and not an individual exon.
   5. Select the Tools Menu.
   6. Select Start ProtAnnot

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   1. .
   • ProtAnnot is launched from IGB.
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   Your selected gene model(s) should look as follows when loaded into ProtAnnot

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   ; take note of the following key items in red

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   • The ProtAnnot menu
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   • The navigation and zoom bar
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   • The selected gene model(s) name
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   • The Properties and InterProScan tabs
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   • The ProtAnnot window title should contain contains the absolute sequence coordinates, strand direction, contig name, and the genome version.
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   1. Select the ProtAnnot

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   1. menu.
   2. Select

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   1. Run InterProScan

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   1. menu item.

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   The InterProScan Job Configuration window

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   • Verify tool tips in the information icon wrap appropriately
   • Verify the link in the highlighted box below links to the appropriate InterProScan about page
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   should look as follows:

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   • Text is wrapping correctly for the information icon.
     
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   • The information link directs to the appropriate "About InterPro" page.
     
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   1. Provide an email address in the Email field.
      1. An email address is required for running InterProScan. Otherwise, InterProScan may not return jobs. 
   2. Click Run.
   3. Select the InterProScan tab.

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   • All job statuses are completed with a status of Finished.
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   • Clicking on the result cell opens the result xml on the InterProScan website.
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   1. Select the Properties tab.
   2. In the display window, select a gene model to open the Properties tab.

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   • Exons have the correct protein sequences.

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   1. In the display window, select a protein annotation to open the Properties tab.
   2. The following properties should be present and correct:

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   • Application and library name are present

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   • • Open the InterProScan tab
       • Verify that that both jobs have a status of FINISHED
       • Clicking on the result cell should open the result xml on the InterProScan website

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   • Run InterProScan again by selection "Run InterProScan"
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   • Functional URL link
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   • Start, end, and length coordinates
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   1. Run the InterProScan again by selecting Run InterProScan under the InterProScan tab.

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   2. Once the jobs are running, select

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   1. Cancel All Jobs

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   1. under the InterProScan tab

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   • Job statuses are "Cancelled" , and that there are no exceptions in the logsthe Log.
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   1. Select ProtAnnot in the top left of the ProtAnnot window, and then choose Save Image...
   2. Save the image to your machine, then open the file.
   • The image is viewable and saved appropriately.
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