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  1. Download and unzip the following folder: test genomes.zip 
  2. The folder "test genomes" should contain the following files:
    1. E_unicornis_Jul_2043.bed.gz.tbi
    2. E_unicornis_Jul_2043.bed.gz
    3. E_unicornis_Jul_2043.2bit
    4. T_rex_Jun_1993.2bit
  3. Start IGB 9.1.8 or later.
  4. Select the Plug-ins tab.
  5. Click Launch App Manager.
  6. Select Manage Repositories...
  7. Click Add...
    1. Name: Quickload Saver Repo
    2. URL: https://bitbucket.org/lorainelab/quickload-saver/downloads/
  8. Click Submit.
  9. Click Done.

  • Checkpoint:
  •  mac
  •  linux
  •  windows


  1. In the Plug-ins tab, click Launch App Manager.
  2. Select the Quickload Saver app.
  3. Click Install.

  • Checkpoint:
  •  mac
  •  linux
  •  windows


  1. Close the IGB App Manager window.
  2. Select File > Open Genome from File...
    1. Reference Sequence: Click Choose File... and select E_unicornis_Jul_2043.2bit
    2. Genus: Equus
    3. Species: unicornis
    4. Variety: (leave blank)
    5. Genome release date: July 2043
  3. Click OK.
  4. Select File > Open File...
  5. Select E_unicornis_Jul_2043.bed.gz
  6. Click Open.
  7. In the Data Access tab at the bottom of IGB,
    1. Change the Load Mode to Genome for E_unicornis_Jul_2043.bed.gz
    2. Change the color of the foreground (FG) to red.

  • Checkpoint:
  •  mac
  •  linux
  •  windows


  1. Select File > Save Custom Genome to Local Quickload Site
  2. Select your desktop as the destination and unicorn as the name.
  3. Click Save.
  4. Select File > Open Genome from File...
    1. Reference Sequence: Click Choose File... and select the T_rex_Jun_1993.2bit
    2. Genus: Tyrannosaurus 
    3. Species: rex
    4. Variety: (leave blank)
    5. Genome release date: June 1993
  5. Click OK.
  6. Select File > Open URL...
  7. Enter URL: http://igbquickload.org/quickload/S_cerevisiae_Apr_2011/S_cerevisiae_Apr_2011_ncbiRefSeq.bed.gz
  8. Click OK.

  • Checkpoint:
  •  mac
  •  linux
  •  windows


In the Data Access tab at the bottom of IGB:

  • Change the Load Mode to Genome for S_cerevisiae_Apr_2011_ncbiRefSeq.bed.gz
  • Change the color of the foreground (FG) to green.

  • Checkpoint:
  •  mac
  •  linux
  •  windows


  1. Select File > Save Custom Genome to Local Quickload Site.
  2. Select your desktop as the destination and rex as the name.
  3. Select File > Preferences...
  4. In the Preferences window select the Other Options tab.
  5. Click Reset Preference to Defaults and then click Yes. IGB will close.
  6. Start IGB.
  7. Select File > Preferences.
  8. Select the Data Sources tab.
  9. Click Add...
    1. Name: unicorn
    2. Type: Quickload
    3. Click Choose local folder
  10. Select the unicorn folder on your desktop and click Open.
  11. Click Submit

  • Checkpoint:
  •  mac
  •  linux
  •  windows


  1. Click Add...
    1. Name: rex
    2. Type: Quickload
  2. Click Choose local folder.
  3. Select the rex folder on your desktop and click Open.
  4. Click Submit.

  • Checkpoint:
  •  mac
  •  linux
  •  windows


  1. Close the Preferences window.
  2. Select the Log tab and click Clear.
  3. Open the E_unicornis_Jul_2043 genome.
  4. In the Data Access tab, E_unicornis_Jul_2043.bed.gz should be set to Load Mode Genome and should be red (FG).

...

  • Your IGB appears to look the same as the above image.
  •  mac
  •  linux
  •  windows



  1. Navigate to: chrXVI:2,618-2,661
  2. Click Load Sequence.

...

  • Your IGB appears to look the same as the above image.
  •  mac
  •  linux
  •  windows



Select the Log tab and check for errors.

  • There are no errors in the Log.
  •  mac
  •  linux
  •  windows


  1. Click Clear in the Log tab.
  2. Open the T_rex_Jun_1993 genome.
  3. In the Data Access tab, S_cerevisiae_Apr_2011_ncbiRefSeq.bed.gz should be set to Load Mode Genome and should be green (FG).

...

  • Your IGB appears to look the same as the above image.
  •  mac
  •  linux
  •  windows



  1. Navigate to: chrXVI:2,618-2,661
  2. Click Load Sequence.

...

  • Your IGB appears to look the same as the above image.
  •  mac
  •  linux
  •  windows



  1. Select the Log tab and check for errors.
  2. Click Clear in the Log tab.
  • There are no errors in the Log.
  •  mac
  •  linux
  •  windows