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The first step to loading data is to choose Species and Genome Version. IGB uses this information to offer data sources with relevant data sets. To set the species and version, select them in the Data Access panel. Read about the Data Access panel. Image Removed Current Genome Panel. Image Added

Alternatively, if you chose to open a file from File > Open File... or Open URL... the file selection widow will permit you to specify species and genome using the drop down menus at the bottom of the window.

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IGB is capable of loading and displaying whole genomes, whole chromosomes or just portions of a data file, depending on the file type. Therefore, although there are many load options, not every file type can be loaded the same way. There is always the option of Don't Load, which will simply not load the individual file but will maintain it in the list.

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  • Whole Genome is primarily used with sequence files; this is the default load that IGB uses for the reference sequences for most model organisms (e.g. TAIR10 mRNA)
  • Whole Chromosome is the standard load mode for most file types. We generally recommend selecting Whole Chromosome, unless it is a very large file (e.g. CoolI1T1.bed, A_thaliana_Jun_2009.fa).an option for some file types. 
  • Region in View is the default Load Mode for IGB. This setting tells IGB to load only the region in view (e.g. CoolI1T1.sm.wig). For large files, such as .bam, .wig or other short read associated files, we strongly recommend that you zoom into a small region of interest, usually about <100Kb depending on your read density, and then refresh the data Load Data to see the data within this region. This is easily accomplished if the reference genome for your species is already loaded (IGB automatically loads the reference sequences for most model organisms).
  • Autoload is an option for BAM and SAM files (e.g. CoolI1T1.sm.bam), which will allow IGB to automatically load the data in view, if the view is zoomed below the Autoload threshold (this is marked with a white arrow in the zoom bar; red box). The Autoload zoom level can be adjusted to your needs. First, set the main view to the zoom level you want. Then, right click in any blank area of the view and select Set Autoload Threshold to Current View. This will change the needed zoom level AND will move the white arrow to indicate the new threshold setting. BE CAREFUL! If you set the Autoload threshold too high, IGB will try to load all of the indicated data, which could exceed the memory limit.

 

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Load Data to Visualize

After the data are set to the appropriate Load ModesModes, simply Refresh Load Data to see the data. If you want all the tracks to display at once, use the Refresh button in one of the Data Access panel or the refrtesh icon at the top of the window. Alternatively, if you want to visualize one track at a time, use the individual refresh icon next to the tracks.Image Removed Load Data buttons; to load a single track use the Load Data icon in the Data Management table. Sequence can be loaded from either Load All Sequence or Load Sequence in View buttons*.*

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You might partially load a track (Region in View, Autoload) and then zoom out to show a greater region of the data sets. IGB will show gray in the area of any track that is not fully loaded.

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