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- Select one or more graphs.
- Click the Thresholding button in the Graph Adjuster tab panel. This will open the Graph Thresholds window.
- Set Visibility to On.
- Modify the thresholding parameters. By default, the threshold is set midway between the graph minimum and maximum values. To obtain more meaningful thresholds, adjust the threshold settings using the methods described in the following sections.
- (Optional) Save the resulting threshold regions to an annotation track by clicking Make Track.
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After you have captured threshold "annotations" from a graph or other set of data, you can examine these "annotations" using all of the tools in IGB for working with and comparing annotations.
Getting details about a graph
Textual information about some graphs is available in Selection Info.
Click the colored bar at the left of the graph, then click the Selection Info tab. Resize the columns if needed.
Saving and bookmarking graphs
Most graph properties that you set in IGB can be captured for future use by saving or bookmarking.
Saving graph data as a file
You can save a graph as a separate file for future reference or off line use. Note that this saves only the graph data, without any visualization customizations you may have made to it.
To save graph data to a file:
- Select the graph by clicking the colored bar at the left end of the graph.
- Click the Graph Adjuster tab. Click the Save button.
- Specify location and graph name.
- Pick one of the graph formats offered. Typically choose ".egr" format for interval graphs and ".sgr" format for other graphs.
- You will be prompted to add the proper ".sgr" or ".egr" filename extension depending on the type of graph data.
- Click OK.
Graph properties, such as color, scale, and so forth, may not be saved along with the data. Some graph formats, such as ".bed", ".gff", and ".wig" allow such properties to be specified in "track lines".
Bookmarking graph views
By creating a bookmark, you can save a link to your graph along with the customizations you have made to its appearance. See Bookmarking on page 75.
You can also export your list of bookmarks to share it with other users. If your bookmarks contain links to graph files on your file system, those files may not be available to users on other file systems.
Printing graphs
To print directly to your printer:
- Choose File menu > Print.
You can also print to an EPS format file. Files in the EPS format print in high-resolution and can be imported into many graphics programs. Files printed in EPS format are suitable for use in publications. To print to an EPS file:\\
- Choose File menu > Export.
- Choose to export either the Main View or Main View (With Labels)
- Choose a name for your file, using the extension ".eps" .
Setting graph preferences
To set preferences that apply by default when you load graphs, see Setting graph preferences on page 76.