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Step 2: Choose species and genome version
Click an image from the a start screen (red box) shortcut image.
or
Choose species and genome version under using the Current Genome tab (blue box) menus.
- If your genome of interest is not listed, don't choose anything and skip to Step 4.
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IGB after selecting the human genome
Step 3. Open data sets
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and click Load Data
Choose data sets from Available Data file tree
Open a folder under the Available Data file tree (red box) by clicking on it.
Select data sets you would like to view by
- checking the box (red arrow)
- click-dragging the data set into the Data Management table
| Info |
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When you open a data set, IGB adds it as new empty track to the main view and lists it in the Data Management table (orange arrows). |
| Note |
The newly-added track is empty at first to give you more control over how much data are loaded into memory. |
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- Use File > Open... or File > Open URL... to select a file or enter a URL
- Click-drag the file or Web link into the IGB main viewer
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To load data into the main view, click Load Data.
| Noteinfo |
|---|
Many data files and data sets (such as BAM files) contain too much data to display at once. For this reason, IGB does not automatically load data from a newly opened data set or file. To gives you total control over when and how data are loaded. To load and view data, zoom in on a region and click the Load Data button. Regions where data have not yet been loaded have slightly darker backgrounds. |
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If you have a sequence file (fasta, 2bit, bnib, etc.) that you would like to use as the reference sequence, use the File > Open Reference Sequence... option. After selecting the file, click the Load Data button to load sequence into sequence. The sequence data will appear below the Coordinate Axis in the center of the display.
Step 5:
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Zooming in on a region of interest
If you're working with large files such as .bam, .sam, .wig, .bedGraph, you should 'zoom in' to a smaller area before loading your data.
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- Use the zoom slider. Click in the main window to focus zooming and then drag the slider to the right to zoom in.
- Search for a gene. Click the Advanced Search tab and enter the name of a gene. Double-click rows in the results list to jump to a feature (red box/arrow).
- See also Searching in IGB
- Double click a feature to zoom in on it (orange box/arrow).
- See also Selecting a feature
- Enter a coordinates in the coordinate box and hit enter to zoom to that location (green box/arrow)
- Click-drag a region in the coordinate axis axix to zoom to in on a region of interest (blue box/arrow).
- Keyboard shortcuts Use control+mouse wheel (Windows) or command+mouse wheel (Mac) to zoom in on the zoom stripe location (yellow box/arrow).
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