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- Click an image or choose species and version under the Current Genome tab (blue box).
- For some species, gene models and cytological bands will automatically load.
- If your genome of interest is not listed, don't choose anything and skip to Step 3.
IGB start screen
IGB after selecting the latest human genome. RefSeq alignments are show in blue.
Step 3. Load data sets from Data Source servers
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Open a data source
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folder under the Data Sources and Data Sets file tree (red box)
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. Select data sets you would like to view by
- checking the box (red arrow)
- or click-dragging them into the Data Management table
When you select a data the data sets you would like to load, IGB will add new track to the main viewer and list the file in the Data Management table (orange box) to the main viewerarrows). Initially the track will be empty. This allows you to zoom in to a smaller region of interest before loading data into the memory.
To view data
- Zoom or scroll to a region of interest
- Click Load Data (green arrowsarrow, boxesbox) to load data into the IGB main view.
Step 4: Load data from local files
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When you open a file, IGB adds new track to the main view .
Areas that are NOT loaded are grayed out (as you can see in the place holder tracks).
(as a gray place holder track) and adds the file to the list of active data sets in the Data Management Table.
To load data into the main view, click Load Data.
Note: Many data files (especially BAM files) are huge and contain too much data to display at once. For this reason, IGB does not NOT automatically load data from a newly opened file. To view data in a file, zoom and scroll to a region or gene of interest and click Load Data. IGB indicates regions that have not yet been loaded by displaying a slightly darker background color.
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If you have a sequence file (fasta, 2bit, bnib, etc.) that you would like to use as the reference sequence, use the File > Open Reference Sequence... option. After selecting the file, click *Load Data* button to load sequence into the Coordinate Axis.
Step 5: Zoom in on a region of interest
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- Use the zoom slider to zoom in on a gene (purple box/arrow). Click in the main window to focus zooming and then drag the slider to right to zoom in.
- Click the Advanced Search tab and enter the name of a gene. Double-click rows in the results list to jump to a feature (red box/arrow).
- See also Searching in IGB
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- Use control+mouse wheel (Windows) or command+mouse wheel (Mac) to zoom in on the zoom stripe location (yellow box/arrow).
See also:
- More about scrolling/panning in IGB
- More about the main view
- More about the tabbed panels and about customizing them.
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To change track appearance
- Right-click (or control-click on Mac) a track label and choose Change or Configure.. options
- Click File > Preferences
- Click a color swatch in the Data Management TableOpen the Annotations panel. This panel allows you to change style, size of the tracks, amount of data shown within the track, as well as offers various functions that can be performed on the track.
See also: