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IGB is capable of loading and displaying whole genomes, whole chromosomes or just portions of a data file, depending on the file type. Therefore, although there are many load options, not every file type can be loaded the same way. There is always the option of Don't Load, which will simply not load the individual file but will maintain it in the list. Image Added

  • Whole Genome is primarily used with sequence files; this is the default load that IGB uses for the reference sequences for most model organisms (e.g. TAIR10 mRNA)
  • Whole Chromosome is the standard load mode for most file types. We generally recommend selecting Whole Chromosome, unless it is a very large file (e.g. CoolI1T1.bed, A_thaliana_Jun_2009.fa).
  • Region in View tells IGB to load only the region in view (e.g. CoolI1T1.sm.wig). For large files, such as BAM .bam, .wig or other short read sequence associated files, we strongly recommend that you zoom into a small region of interest, usually about <100Kb depending on your read density, and then refresh the data to see the data within this region. This is easily accomplished if the reference genome for your species is already loaded (IGB automatically loads the reference sequences for most model organisms).
  • Autoload is a new option in IGB6.5. This is an option for BAM files.bam files (e.g. CoolI1T1.sm.bam), which will allow IGB to automatically load the data in view if the view is zoomed below the Autoload threshold (this is marked with a white arrow in the zoom bar; see below).

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